课题组聚焦合成生物学、基因编辑领域关键科学问题，解析基因编辑元件作用的分子机制，开发高精度基因编辑核酸酶，实现高精度、高效率的基因编辑；开发高通量基因组编辑策略，对理性或半理性设计的生物元件进行高通量构建、筛选以及基因型-表型的快速表征，实现对生物元器件的进化规律、催化原理、相互作用机制的系统性研究；在此基础上，将开发的合成生物学技术策略应用于构造具有特定功能的细胞工厂，实现快速、高效合成生物基化学品、生物燃料以及生物医疗产品等，为实现合成生物学高效构建细胞工厂提供理论基础及实践范例。

主要研究方向：（1）高精度CRISPR基因编辑核酸酶的开发与应用；（2）高通量CRISPR基因组编辑工程策略开发与应用；（3）细胞工厂的功能预测与智能化设计；（4）构建细胞工厂合成生物基化学品、生物燃料以及高附加值产品。

团队发表近60篇国内外学术知名期刊，包括 Science、Nature Biotechnology、 Nature Communications、Trends in Biotechnoloy、 Metabolic Engineering、 Bioresource Technology 及ACS Synthetic Biology等领域内权威期刊，编写英文专著2篇。引用达到 2000 余次，H-index 为 27；申请及授权发明专利 10 余项，PCT专利 5 项。刘嵘明教授入选国家级青年人才项目、辽宁省兴辽英才计划青年拔尖人才、大连市高端人才及大连市引进中国籍海外高层次人才项目，梁丽亚副教授入选大连市紧缺人才，团队成员主持科技部重点研发专项课题及子课题，国家自然科学基金项目（面上及青年）、国家级青年人才计划（海外）项目、辽宁省自然科学基金面上项目、大连市科技创新基金项目、医工交叉项目及中央高校基本科研业务费，参与多项美国能源部项目（DOE）项目、美国国防先进研究计划局（DARPA）项目并主持江苏省普通高校研究生科技创新计划项目3项。担任 Frontiers in Bioengineering and Biotechnology杂志的客座编辑以及主题编辑，Frontiers in Microbiology 杂志的评审编辑，审稿专业期刊文章 60 余篇。获得江苏省优秀博士毕业论文及江苏省优秀学生干部。多次参加国内外学术会议，并于第一届、第二届先进生物制造国际会议、美国工业微生物与生物技术年会（SIMB)及2019年美国化学会年会（2019 ACS National Meeting & Expo）做口头报告。指导学生获得2022、2023、2024年国际基因工程大赛（iGEM）2项金奖、1项银奖及3项单项提名奖。指导大学生获得优秀本科生基础研究项目2项、创新创业项目并获得国家级立项3项，指导大学生获得生命科学竞赛全国二等奖2项、省赛一等奖2项及合成生物学竞赛金奖1项。

主持或参与科研项目情况：

1. 国家级青年人才计划（海外）项目，2022-2024，主持

2. 科技部重点研发专项课题，2024-2028，主持

3. 科技部重点研发专项子课题，2024-2028，主持

4. 国家自然科学基金面上项目, 2023.01-2026.12，主持

5. 国家自然科学基金青年项目, 2023.01-2025.12，主持

6. 辽宁省兴辽英才计划项目，2023-2025，主持

7. 辽宁省自然科学基金项目，2024-2026，主持

8. 大连市科技创新基金项目，2022-2024，主持

9. 中央高校基本科研业务费1, 2022-2023，主持

10. 中央高校基本科研业务费2, 2022-2023，主持

11.医工交叉项目，2024-2025，主持

12.AI4S交叉项目，2024-2025，主持

13.横向项目1,2024-2025，主持

14.横向项目2,2024.01-2024.12，主持

15.大连理工大学学科建设高层次人才引育专项, 2022-2024，主持

16.大连市引进中国籍海外高层次人才项目青年项目, 2022，主持

17.美国能源部DOE项目, 2018-2020，项目骨干

18.美国能源部DOE项目, 2015-2016，项目骨干

19.美国能源部DOE项目, 2014-2017，项目骨干

20.美国国防先进研究计划局DARPA项目, 2017-2020，项目骨干

21.江苏省普通高校研究生科技创新计划, 2013-2014，主持

22.江苏省普通高校研究生科技创新计划, 2012-2013，主持

23.江苏省普通高校研究生科技创新计划, 2011-2012，主持

Selected Publications (*通讯作者)

(1) Rongming Liu; Liya Liang; Emily F. Freed; Alaksh Choudhury; Carrie A. Eckert; Ryan T. Gill*. Engineering regulatory networks for complex phenotypes in E. coli. Nature Communications, 2020, 11, 4050.

(2) Rongming Liu*; Liya Liang; Emily F. Freed; Ryan T. Gill*. Directed evolution of CRISPR/Cas systems for precise gene editing. Trends in Biotechnology, 2020, 39(3): 262-273.

(3) Rongming Liu; Liya Liang; Emily Freed; Hao Chang; Eun Joong Oh; Zeyu Liu; Andrew Garst; Carrie A. Eckert; Ryan T. Gill*. Synthetic chimeric nucleases function for efficient genome editing. Nature Communications, 2019, 10, 5524.

(4) Liya Liang; Rongming Liu; Kyle E.O. Foster; Alaksh Choudhury; Sherri Cook; Jeffrey C. Cameron; Wil V. Srubar III; Ryan T. Gill*. Genome engineering of E. coli for improved styrene production. Metabolic Engineering, 2019, 57: 74-84.

(5) Rongming Liu; Liya Liang; Andrew D. Garst; Alaksh Choudhury; Violeta Sànchez i Nogué; Gregg T. Beckham; Ryan T. Gill*. Directed combinatorial mutagenesis of Escherichia coli for complex phenotype engineering. Metabolic Engineering, 2018, 47: 10-20.

(6) Rongming Liu; Liya Liang; Alaksh Choudhury; Marcelo C. Bassalo; Andrew D. Garst; Katia Tarasava; Ryan T. Gill*. Iterative genome editing of Escherichia coli for 3-hydroxypropionic acid production. Metabolic Engineering, 2018, 47: 303-313.

(7) Rongming Liu; Liya Liang; Alaksh Choudhury; Andrew D. Garst; Carrie A. Eckert; Eun Joong Oh; James Winkler; Ryan T. Gill*. Multiplex navigation of global regulatory networks (MINR) in yeast for improved ethanol tolerance and production. Metabolic Engineering, 2018, 51: 50-58.

(8) Andrew D Garst; Marcelo C Bassalo; Gur Pines; Sean A Lynch; Andrea L Halweg-Edwards; Rongming Liu; Liya Liang; Zhiwen Wang; Ramsey Zeitoun; William G Alexander; Ryan T Gill*. Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering. Nature Biotechnology, 2017 35, 48-55.

(9) Rongming Liu; Marcelo C. Bassalo; Ramsey I. Zeitoun; Ryan T. Gill*. Genome scale engineering techniques for metabolic engineering. Metabolic Engineering, 2015, 32: 143-154.

(10) Rongming Liu; Liya Liang; Feng Li; Mingke Wu; Kequan Chen; Jiangfeng Ma; Min Jiang*; Ping Wei; Pingkai Ouyang. Efficient succinic acid production from lignocellulosic biomass by simultaneous utilization of glucose and xylose in engineered Escherichia coli. Bioresource Technology, 2013, 149: 84-91.

1. 刘嵘明，梁丽亚，一种提高CRISPR/Cas基因编辑效率的方法，中国发明专利号：ZL202210981325.7

2.刘嵘明，梁丽亚，樊祥瑞，一种高效构建gRNA的方法，中国发明专利，公开号：CN117070546A

3. Jamie Kershner, Rongming Liu, Liya Liang, Roland Baumgartner, Tanya Warnecke. Constructs and uses thereof for efficient 

and specific genome editing, PCT/US21/51142,国际发明PCT专利, 美国发明专利

4. Ryan Gill, Rongming Liu, Engineered Chimeric Nucleic Acid Guided Nucleases Compositions, Methods for Making, and Systems for Gene Editing, PCT/US2019/054869, 国际发明PCT专利, 美国发明专利

5. Ryan Gill, Rongming Liu, Engineered Chimeric Nucleic Acid Guided Nuclease Constructs and Uses Thereof, PCT/US2019/054872, 国际发明PCT专利, 美国发明专利

6. Min Jiang, Rongming Liu, Liya Liang, Jiangfeng Ma, Kequan Chen, Ping Wei, Construction method of Escherichia coli genetically engineered bacteria producing succinic acid by xylose metabolism, PCT/CN2012/078817, 国际发明PCT专利, 美国发明专利

7. Min Jiang, Min Zhang, Jiangfeng Ma, Hao Wu, Liya Liang, Rongming Liu, Guangming Wang Ping Wei, Method for reusing water in fermented butanedioic acid separation process, US9562242B2, 美国发明专利

8. 姜岷，刘嵘明，梁丽亚，吴明科，曹伟佳，马江锋，陈可泉，韦萍，一株产丁二酸基因工程菌及其发酵生产丁二酸的方法, 中国发明专利号：ZL201210143173.X

9. 姜岷，刘嵘明，梁丽亚，吴明科，曹伟佳，马江锋，陈可泉，韦萍，一株产丁二酸基因工程菌及其发酵生产丁二酸的方法, 中国发明专利号：ZL201110380396.3

10. 姜岷，刘嵘明，梁丽亚，包海姣，马江锋，陈可泉，韦萍，一株厌氧利用合成培养基高产丁二酸大肠杆菌的筛选及其应用, 中国发明专利号：ZL201310288669.0

11. 姜岷，梁丽亚，刘嵘明，万青，陈旭，任心怡，马江锋，陈可泉，韦萍，欧阳平凯，一株大肠埃希氏菌LL016及其应用, 中国发明专利号：ZL201210515218.1

12. 姜岷，梁丽亚，刘嵘明，马江锋 陈可泉，韦萍，利用大肠埃希氏菌BA305发酵生产丁二酸的方法, 中国发明专利号：ZL201210142752.2

13. 姜岷，吴明科，刘嵘明，梁丽亚，马江锋，陈可泉，韦萍，欧阳平凯，一株铵根离子耐受型产丁二酸大肠杆菌及其应用, 中国发明专利号：ZL201310279778.6

14. 姜岷，包海姣，刘嵘明，梁丽亚，马江锋，陈可泉，韦萍，一株厌氧利用合成培养基高产丁二酸大肠杆菌的筛选及其应用, 中国发明专利号：ZL201310288538.2

15. 姜岷，李凤，刘嵘明，梁丽亚，马江锋，陈可泉，韦萍，欧阳平凯，一株利用蔗糖产丁二酸基因工程菌株及其发酵生产丁二酸的方法, 中国发明专利号：ZL201410099403.6

16. 姜岷，张汉文，马江锋，刘嵘明，梁丽亚，一种利用厌氧条件下发酵生产丁二酸的方法, 中国发明专利号：ZL201410212929.0

17. 姜岷，王光明，马江锋，梁丽亚，刘嵘明，张敏，一种两阶段发酵生产苹果酸的方法, 中国发明专利号：ZL201110309507.1

18. 姜岷，张常青，苟冬梅，梅佳军，刘嵘明，马江锋，吴明科，曹伟佳，利用合成培养基纯厌氧生长产丁二酸的大肠杆及其应用, 中国发明专利号：ZL201210138292.6

19. 姜岷，万青，张常青，梁丽亚，陈旭，苟冬梅，刘嵘明，马江锋，产丁二酸的菌株及其生产丁二酸的方法和应用, 中国发明专利号：ZL201210392035.5

20. 姜岷，陈旭，梁丽亚，万青，苟冬梅，刘嵘明，马江锋，产丁二酸基因工程菌及其构建及应用, 中国发明专利号：ZL201210392834.2

SCI文章汇总：

1. Liya Liang, Huiping Tan, Rongming Liu*. Emerging gene editing in industrial microbiology beyond CRISPR-Cas9. Trends in Biotechnology, 2024. (IF= 19.536), 工程技术1区 Top期刊

2. Ranfeng Wu, Yiming Chen, Yongpeng Zhang, Rongming Liu, Qiang Zhang*, Cheng Zhang*. Catalytic Gold Nanoparticle Assembly Programmed by DNAzyme Circuits. Small 2024, 20, 2307107.

       3. Xin Liu,  Xun Zhang, Shuang Cui, Shujuan Xu, Rongming Liu, Bin Wang, Xiaopeng Wei, Qiang Zhang*. A signal transmission 

        strategy driven by gap-regulated exonuclease hydrolysis for hierarchical molecular networks. Communications Biology,  2024, 7:       335.

4. Rongming Liu*, Liya Liang, Emily F. Freed, Ryan T. Gill*. Directed evolution of CRISPR/Cas systems for precise gene editing. Trends in Biotechnology, 2021, 39(3):262-273. (IF= 19.536), 工程技术1区 Top期刊

5. Liya Liang, Rongming Liu, Emily F. Freed, Carrie A. Eckert, and Ryan T. Gill*. Transcriptional regulatory networks involved in C3-C4 alcohol stress response and tolerance in yeast. 2020, ACS Synthetic Biology, 2021, 10, 1, 19–28.

6. Rongming Liu, Liya Liang, Emily F. Freed, Alaksh Choudhury, Carrie A. Eckert, Ryan T. Gill*. Engineering regulatory networks for complex phenotypes in E. coli. Nature Communications, 2020, 11(1): 4050 (IF= 14.919), 综合性期刊 1区 Top期刊

7. Liya Liang, Rongming Liu, Kyle E.O. Foster, Alaksh Choudhury, Sherri Cook, Jeffrey C. Cameron, Wil V. Srubar III, Ryan T. Gill*. Genome engineering of E. coli for improved styrene tolerance and production. Metabolic Engineering, 2020,57:74-84 (IF=9.783) 工程技术1区Top期刊

8. Liya Liang, Rongming Liu, Emily F. Freed, Carrie A. Eckert*. Synthetic biology and metabolic engineering Employing Escherichia coli for C2-C6 bioalcohol production. Frontiers in Bioengineering and Biotechnology, 2020, 8:710.

9. Eun Joong Oh, Rongming Liu, Liya Liang, Emily F. Freed, Carrie A. Eckert, Ryan T. Gill*. Multiplex evolution of antibody fragments utilizing a yeast surface display platform. ACS Synthetic Biology, 2020, 9, 8, 2197–2202.

10. Rongming Liu, Liya Liang, Emily Freed, Hao Chang, Eun Joong Oh, Zeyu Liu, Andrew Garst, Carrie A. Eckert, Ryan T. Gill*. Synthetic chimeric nucleases function for efficient genome editing, Nature Communications, 2019, 10(1):5524, (IF= 14.919), 综合性期刊 1区 Top期刊

11. Rongming Liu, Liya Liang, Alaksh Choudhury, Andrew Garst, Carrie A. Eckert, Oh Eun Joong, James Winkler, Ryan T. Gill*. Multiplex navigation of global regulatory networks (MINR) in yeast for improved ethanol tolerance and production. Metabolic Engineering, 2019, 51: 50-58. (IF=9.783) 工程技术1区Top期刊

12. Rongming Liu, Liya Liang, Alaksh Choudhury, Marcelo C. Bassalo, Andrew D. Garst, Katia Tarasava, Ryan T. Gill*. Iterative genome editing of Escherichia coli for 3-hydroxypropionic acid production. Metabolic Engineering, 2018, 47:303-313. (IF=9.783) 工程技术1区Top期刊

13. Rongming Liu, Liya Liang, Andrew D. Garst, Alaksh Choudhury, Violeta Sànchez iNogué, Gregg T. Beckham, Ryan T. Gill*. Directed combinatorial mutagenesis of Escherichia coli for complex phenotype engineering. Metabolic Engineering, 2018, 47:10-20. (IF=9.783) 工程技术1区Top期刊

14. Liya Liang, Chelsea M. Heveran, Rongming Liu, Ryan T. Gill, Aparna Nagarajan, Jeffrey Cameron, Mija Hubler, Wil V. Srubar III, Sherri M. Cook*. Rational Control of Calcium Carbonate Precipitation by Engineered Escherichia coli. ACS synthetic Biology, 2018, 7(11):2497-2506.

15. Katia Tarasava, Rongming Liu, Ryan T. Gill, Andrew Garst. Combinatorial pathway engineering using type I‐E CRISPR interference. Biotechnology and bioengineering, 2018, 115:1878-1883.

16. Andrew D Garst, Marcelo C Bassalo, Gur Pines, Sean A Lynch, Andrea L Halweg-Edwards, Rongming Liu, Liya Liang, Zhiwen Wang, Ramsey Zeitoun, William G Alexander, Ryan T Gill*. Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering. Nature Biotechnology, 2017, 35:48-55. (IF=31.865)

17. Eric M Karp, Todd R Eaton, Violeta Sànchez i Nogué, Vassili Vorotnikov, Mary J Biddy, Eric CD Tan, David G Brandner, Robin M Cywar, Rongming Liu, Lorenz P. Manker, William E, ichener, Michelle Gilhespy, Zinovia Skoufa, Michael J. Watson, O Stanley Fruchey, Derek R.Vardon, Ryan T, Gill, Adam D, Bratis, Gregg T. Beckham. Renewable acrylonitrile production. Science, 2017, 358(6368):1307-1310.

18. Liya Liang, Rongming Liu, Andrew D. Garst, Thomas Lee, Violeta Sànchez i. Nogué, Gregg T. Beckham, Ryan T. Gill*. CRISPR EnAbled Trackable genome Engineering for isopropanol production in Escherichia coli. Metabolic engineering, 2017, 41:1-10.

19. Min Jiang, Jiangfeng Ma, Mingke Wu, Rongming Liu, Liya Liang, Fengxue Xin, Wenming Zhang, Honghua Jia, Weiliang Dong. Progress of succinic acid production from renewable resources: metabolic and fermentative strategies. Bioresource Technology, 2017, 245(B):1710-1717.

20. Marcelo C. Bassalo, Rongming Liu, Ryan T. Gill. Directed evolution and synthetic biology applications to microbial systems. Current Opinion in Biotechnology, 2016, 39:126-133.

21. Rongming Liu, Marcelo C. Bassalo, Ramsey I. Zeitoun, Ryan T. Gill*. Genome scale engineering techniques for metabolic engineering. Metabolic Engineering, 2015, 32:143-15. (IF= 9.783) 工程技术1区Top期刊

22. Haijiao Bao, Rongming Liu, Liya Liang, Youming Jiang, Min Jiang, Jiangfeng Ma, Kequan Chen, Honghua Jia, Ping Wei, Pingkai Ouyang. Succinic acid production from hemicellulose hydrolysate by an Escherichia coli mutant obtained by atmospheric and room temperature plasma and adaptive evolution. Enzyme and Microbial Technology, 2014, 66:10-15.

23. Rongming Liu, Liya Liang, Min Jiang*, Jiangfeng Ma, Kequan Chen, Honghua Jia, Ping Wei, Pingkai Ouyang. Effects of redox potential control on succinic acid production by engineered Escherichia coli under anaerobic conditions. Process Biochemistry, 2014, 49:740-744.

24. Jiangfeng Ma, Feng Li, Rongming Liu, Liya Liang, Yaliang Ji, Ce Wei, Min Jiang, Honghua Jia, Pingkai Ouyang. Succinic acid production from sucrose and molasses by metabolically engineered E. coli using a cell surface display system. Biochemical Engineering Journal, 2014, 91:240-249.

25. Rongming Liu, Liya Liang, Weijia Cao, Mingke Wu, Kequan Chen, Jiangfeng Ma, Min Jiang*, Ping Wei, Pingkai Ouyang. Succinate production by metabolically engineered Escherichia coli using sugarcane bagasse hydrolysate as the carbon source. Bioresource Technology, 2013, 135:574-577. (IF=9.642) 工程技术1区期刊

26. Rongming Liu, Liya Liang, Feng Li, Mingke Wu, Kequan Chen, Jiangfeng Ma, Min Jiang*, Ping Wei, Pingkai Ouyang. Efficient succinic acid production from lignocellulosic biomass by simultaneous utilization of glucose and xylose in engineered Escherichia coli. Bioresource Technology, 2013, 149:84-91. (IF=9.642) 工程技术1区期刊

27. Rongming Liu, Liya Liang, Mingke Wu, Kequan Chen, Min Jiang*, Jiangfeng Ma, Ping Wei, Pingkai Ouyang. CO₂ fixation for succinic acid production by engineered Escherichia coli co-expressing pyruvate carboxylase and nicotinic acid phosphoribosyl transferase. Biochemical Engineering Journal, 2013,79:77-83.

28. Rongming Liu, Liya Liang, Jiangfeng Ma, Xinyi Ren, Min Jiang*, Kequan Chen, Ping Wei, Pingkai Ouyang. An engineering Escherichia coli mutant with high succinic acid production in the defined medium obtained by the atmospheric and room temperature plasma. Process Biochemistry, 2013,48:1603-1609.

29. Liya Liang, Rongming Liu, Xu Chen, Xinyi Ren, Jiangfeng Ma, Kequan Chen, Min Jiang, Ping Wei, Pingkai Ouyang. Effects of overexpression of NAPRTase, NAMNAT, and NAD synthetase in the NAD(H) biosynthetic pathways on the NAD(H) pool, NADH/NAD⁺ ratio, and succinic acid production with different carbon sources by metabolically engineered Escherichia coli. Biochemical Engineering Journal, 2013, 81, 90-96.

30. Liya Liang, Rongming Liu, Feng Li, Mingke Wu, Kequan Chen, Jiangfeng Ma, Min Jiang, Ping Wei, Pingkai Ouyang. Repetitive succinic acid production from lignocellulose hydrolysates by enhancement of ATP supply in metabolically engineered Escherichia coli. Bioresource Technology, 2013, 143:405-412.

31. Jiangfeng Ma, Dongmei Gou, Liya Liang, Rongming Liu, Xu Chen, Changqing Zhang, Jiuhua Zhang, Kequan Chen, Min Jiang. Enhancement of succinate production by metabolically engineered Escherichia coli with co-expression of nicotinic acid phosphoribosyltransferase and pyruvate carboxylase. Applied Microbiology and Biotechnology, 2013, 97:6739-6747.

32. Min Jiang, Qing Wan, Rongming Liu, Liya Liang, Xu Chen, Mingke Wu, Hanwen Zhang, Kequan Chen, Jiangfeng Ma, Wei Ping. Succinic acid production from corn stalk hydrolysate in an E. coli mutant generated by atmospheric and room-temperature plasmas and metabolic evolution strategies. Journal of Industrial Microbiology & Biotechnology, 2013, Doi:10.1007/s10295-013-1346-7.

33. Liya Liang, Rongming Liu, Guangming Wang, Dongmei Gou, Jiangfeng Ma, Kequan Chen, Min Jiang, Ping Wei, Pingkai Ouyang. Regulation of NAD(H) pool and NADH/NAD⁺ ratio by overexpression of nicotinic acid phosphoribosyltransferase for succinic acid production in Escherichia coli NZN111. Enzyme and Microbial Technology, 2012, 51:286-293.

34. Rongming Liu, Liya Liang, Jiangfeng Ma, Kequan Chen, Min Jiang*. Fermentation of xylose to succinate by enhanced of ATP supply in metabolically engineered Escherichia coli. Applied Microbiology and Biotechnology, 2012, 94(4):959-968.

35. Liya Liang, Rongming Liu, Jiangfeng Ma, Kequan Chen, Min Jiang, Ping Wei. Increased production of succinic acid in Escherichia coli by overexpression of malate dehydrogenase. Biotechnology Letters, 2011, 33:2439-2444.

EI及中文期刊汇总：

   36.刘嵘明**，谭慧萍，王晴晴，张皓喃，孜力汗，梁丽亚。基于CRISPR-Cas系统的微生物基因编辑与调控技术。微生   物学杂志，2024，44（6）：1-14.

   37.赫庆雯，丁鑫垚，梁丽亚*，刘嵘明*。微生物合成3-羟基丙酸的研究进展。生物加工过程，2024，22（6）

   38. 梁丽亚，刘嵘明*。靶向DNA的Ⅱ类CRISPR/Cas系统的蛋白工程化改造。合成生物学，2023，4（1）：1-16.

   39. 樊祥瑞，王俊燕，梁丽亚，刘嵘明。基于CRISPR/Cas系统的多重基因编辑与调控技术。生物工程学报，2023, 39(6): 2449-2464.

   40. 刘嵘明，马江锋，梁丽亚，徐冰，王光明，张敏，姜岷。过量表达烟酸转磷酸核糖激酶对大肠杆菌NZN111产丁二酸的影响。生物工程学报，2011，27（10），1438-1447.

41. 刘嵘明，梁丽亚，吴明科，姜岷。微生物发酵生产丁二酸研究进展。生物工程学报，2013，10:1386-1397.

42. 刘嵘明，梁丽亚，王光明，张敏，马江锋，陈可泉，姜岷，韦萍。基于辅因子调控对大肠杆菌两阶段发酵产丁二酸的影响。生物加工过程，2012, (03): 1-5.

43. 苟冬梅，梁丽亚，刘嵘明，张常青，吴明科，马江锋，陈可泉，朱建国，姜岷。过量表达烟酸单核苷酸腺苷酰转移酶对大肠杆菌NZN111产丁二酸的影响。生物工程学报， 2011，9：1059-1069.

44.  梁丽亚，马江锋，刘嵘明，王光明，徐冰，张敏，姜岷。过量表达苹果酸脱氢酶对大肠杆菌NZN111产丁二酸的影响。生物工程学报，2011，27（7）：1005-1012.

45. 马江锋，梁丽亚，刘嵘明，张敏，陈可泉，姜岷，韦萍。基于关键酶表达的发酵调控与分子改造策略对E. coli产丁二酸的影响。南京工业大学学报(自然科学版)，2011，3（33）：58-61.

46. 张敏，马江锋，徐冰，梁丽亚，刘嵘明，王光明，姜岷。利用木薯淀粉为原料发酵生产丁二酸的研究。中国酿造，2011，07：29-32.

47. 万青，曹伟佳，张常青，刘嵘明，梁丽亚，陈可泉，马江锋，姜岷。常压室温等离子体诱变高效利用木糖产丁二酸菌株。生物工程学报，2013，11：1692-1695.

48. 陈旭，梁丽亚，刘嵘明，万青，吴明科，姜岷。共表达磷酸烯醇式丙酮酸羧激酶和烟酸转磷酸核糖激酶提高重组大肠杆菌发酵木糖产丁二酸。生物加工过程，2015，1：17-22.

49. 吴明科，刘嵘明，梁丽亚，马江锋，陈可泉，姜岷。大肠杆菌AFP111菌体回收连续转化生产丁二酸。生物工程学报，2013，12：1875-1879.

50. 曹伟佳，苟冬梅，梁丽亚，刘嵘明，陈可泉，马江锋，姜岷。烟酸转磷酸核糖激酶和丙酮酸羧化酶共表达对大肠杆菌BA002产丁二酸的影响。生物工程学报，2013，12：1855-1859.

51. 徐冰，马江锋，梁丽亚，刘嵘明，王光明，张敏，姜岷。基于高密度培养的反复分批发酵法生产丁二酸。化工学报，2011，9：2595-2599.

52. 梁丽亚，刘嵘明，苟冬梅，陈旭，马江锋，陈可泉，姜岷，韦萍。共表达烟酸转磷酸核糖激酶和丙酮酸羧化酶对大肠杆菌NZN111产丁二酸的影响。生物加工过程，2014，4：49-54.

53. 梅佳军，张常青，刘嵘明，马江锋，陈可泉，姜岷。利用改性载体固定化大肠杆菌产琥珀酸。化工进展，2013，1：161-165.

54. 侯顾伟，马江锋，隋姗姗，徐冰，刘嵘明，姜岷。蔗糖磷酸化酶的分离纯化及其催化合成α-熊果苷的研究。生物技术通报，2011，6：182-186.

55. 王光明，马江锋，梁丽亚，刘嵘明，徐冰，姜岷。敲除富马酸酶基因对E.coli厌氧混合酸发酵的影响。生物加工过程，2012，11：46-50.

56. 张常青，苟冬梅，梅佳军，刘嵘明，马江锋，陈可泉，朱建国，姜岷。进化代谢选育高渗透压耐受型产琥珀酸大肠杆菌。生物工程学报，2012，11：1337-1345.

57. 张汉文，刘嵘明，梁丽亚，姜岷，马江锋。大肠杆菌AFP111利用玉米粉全水解液厌氧发酵合成丁二酸。生物加工过程，2015，3：14-19.

1. Rongming Liu, Liya Liang, Sean Stettner, Emily F Freed, Carrie A Eckert. CRISPR-based tools for microbial cell factories, 2021 Microbial Cell Factories Engineering for Production of Biomolecules, page 95-113.

2. RongmingLiu, LiyaLiang, Maria Priscila Lacerda, Emily F.Freed, Carrie A.Eckert. Advances in protein engineering and its application in synthetic biology, 2022, New Frontiers and Applications of Synthetic Biology, page 147-158.

3. Dongdong Yang, Jake Flood, Rongming Liu, Susu He. Design of synthetic biology for the detection of microorganisms., 2022, New Frontiers and Applications of Synthetic Biology, page 231-249.

刘嵘明 博士 教授

办公地点：大连理工大学生物工程学院615室

E-mail:rongming_liu@dlut.edu.cn

梁丽亚 博士 副教授

办公地点：大连理工大学生物工程学院615室

E-mail:liya_liang@dlut.edu.cn