“生物转化与生物分离”课题组现有师生四十多人,其中教授1人,副教授3人。研究工作主要集中于生物催化转化和高效分离技术,重点是利用代谢工程和系统生物学手段进行工业微生物的选育、发酵与分离过程的集成优化等,产品包括1,3-丙二醇、乳酸、2,3-丁二醇、微生物次生代谢产物、天然和海洋活性物质等。
主要研究方向包括:生物质能源和生物基化学品、海洋活性物质的分离与活性研究、蛋白类的分离与修饰耦合集成技术、天然产物的生物转化与分离、天然产物防治糖尿病及其作用机制、微生物来源的先导化合物发现等。先后完成国家自然科学基金、国家重点科技攻关项目、国家863项目、973子课题、教育部新世纪优秀人才支持计划和企业委托工程技术开发等40余项课题,在国内外学术期刊上发表论文462篇,已授权中国专利61项,美国专利1项,主编、参编、主译教材、专著16部,获辽宁省技术发明二等奖1项、自然科学三等奖2项。
一、微生物发酵法生产1,3-丙二醇
二、微生物发酵法生产乳酸
三、生物法生产2,3-丁二醇和乙偶姻
四、天然产物的生物转化与分离
五、海洋活性物质的分离与活性研究
六、微生物来源的先导化合物
七、天然产物防治糖尿病及其作用机制
八、蛋白质的分离与耦合集成技术
九、两步盐析萃取高效分离纯化噬菌体
十、微生物菌群培养技术
[1]一种具有协同作用的α-糖苷酶抑制剂组合物及其应用
[2]一种基于生长耦联的底物流加发酵生产1,3-丙二醇的方法
[3]一种微生物转化法制备属于皂素的方法
[4]一种改善高脂高糖饮食并发症的药物组合物及其应用
[5]一种硅酸盐催化制备生物柴油的方法
[6]一种从克雷伯氏杆菌发酵液中提取1,3-丙二醇的方法
[7]一种用平贝母生产白酒或果酒的方法
[8]一种混菌发酵甘油生产1,3-丙二醇的方法
[9]一种中药保健酒的制备方法
[10]萃取与发酵耦联分离发酵液中2,3-丁二醇的方法
[11]一种从制备海藻酸钙微胶囊的废弃物中回收蛋白的方法
[12]1,3-丙二醇氧化还原酶辅酶特异性设计、表达与应用
[13]一种二元醇与有机酸联产和分离的方法
[14]Method for salting-out extraction of acetone and butanol from a fermentation broth
[15]盐析萃取海参中有效成份的方法
[16]一种以文冠果为原料多产物联产的方法
[17]一种二元醇和有机酸联产和分离方法
[18]一种偶联生产生物柴油和1,3-丙二醇的方法
[19]一种利用高酸值废弃油脂生产生物柴油的方法
[20]一种用微生物将白藜芦醇苷转化为白藜芦醇的方法
[21]一种提取纯化丹酚酸B的方法
[22]大气压冷等离子体诱变微生物的方法
[23]硫酸铵盐析法从微生物发酵液中提取分离1,3-丙二醇
[24]一种从发酵液中分离1,3-丙二醇的双水相萃取方法
[25]一种从发酵液中分离2,3-丁二醇的双水相萃取方法
[26]煅烧硅酸钠催化制备生物柴油
[27]一种利用阴离子交换柱辅助聚乙二醇修饰水蛭素的方法
[28]一种以菊芋为原料发酵生产2,3-丁二醇的方法
[29]碱催化剂强化超临界甲醇法实现低压快速制备生物柴油的方法
[30]一种以富含淀粉中药材为原料发酵生产2,3-丁二醇的方法
[31]一种醛还原酶的重组表达及其在甘油生物转化为1,3-丙二醇中的应用
[32]一种酵母来源重组蛋白发酵液的双水相固液分离方法
[33]一种新型双水相萃取越桔中花青素的方法
[34]一种以菊芋块茎及茎叶为原料发酵生产2,3-丁二醇的方法
[35]一种三液相萃取天然产物有效成分的方法
[36]一种双水相萃取分离蛋白质和酶的方法
[37]一种从发酵液中盐析萃取二元醇耦合回收二氧化碳的方法
[38]一种催化强化超临界甲醇制备生物柴油的方法
[39]一种微氧-厌氧连续流加甘油多罐串联发酵生产1,3-丙二醇的方法
[40]制备发酵型海参蓝莓保健酒的方法
[41]盐析萃取发酵液中丙酮和丁醇的方法
[42]一种盐析萃取发酵液中有机酸的方法
[43]一种重组人血清白蛋白的分离纯化方法
[44]一种糖苷酶催化与盐析萃取偶联制备天然药物的方法
[45]一种具有生物转化特性的哈茨木霉及其应用
[46]一种降糖药物组合物及其应用
[47]一种预防糖尿病药物组合物及其应用
[48]利用微反应器连续合成生物柴油的方法
[49]一株稳定高产2,3-丁二醇的细菌及利用低温等离子体和硫酸二乙酯复合诱变的方法
[50]一种复合催化剂的制备方法及应用
[51]提取南极磷虾油脂及分离生物活性物质的方法
[52]一种萃取与发酵偶联分离发酵液中乳酸的方法
[53]萃取与发酵偶联分离发酵液中乙醇的方法
[54]萃取与发酵偶联分离发酵液中1,3-丙二醇的方法
近3年代表性论文:
[1] Dai, Jian-Ying, Yang, Yu, Dong, Yue-Sheng, Xiu, Zhi-Long. Solid-state co-cultivation of Bacillus subtilis, Bacillus mucilaginosus, and Paecilomyces lilacinus using tobacco waste residue[J], APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2020, 190(3): 1092-1105.
[2] Yang Xiaozhe, Hu Wenzhong, Xiu Zhilong, Jiang Aili, Yang Xiangyan, Sarengaowa,Ji Yaru, Guan Yuge, Feng Ke. Microbial dynamics and volatilome profiles during the fermentation of Chinese northeast sauerkraut by Leuconostoc mesenteroides ORC 2 and Lactobacillus plantarum HBUAS 51041 under different salt concentrations[J], Food research international (Ottawa, Ont.), 2020, 130: 108926
[3] Hou, Mengyang, Hu, Wenzhong, Xiu, Zhilong, Shi, Yusheng, Hao, Kexin,Cao, Duo, Guan, Yuge, Yin, Hanlin. Efficient enrichment of total flavonoids from Pteris ensiformis Burm. extracts by macroporous adsorption resins and in vitro evaluation of antioxidant and antiproliferative activities[J], JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2020, 1138: 121960
[4] Sun, Yaqin, Zhang, Xingxing, Zheng, Yafeng, Yan, Ling, Xiu, Zhilong. Sugaring-out extraction combining crystallization for recovery of succinic acid[J], SEPARATION AND PURIFICATION TECHNOLOGY, 2019, 209:972-983.
[5] Sun, Yaqin, Zheng, Yafeng, Zhou, Jinjie, Xiu, Zhilong. Fermentation perfermance and mechanism of a novel microbial consortium DUT08 for 1,3-propandiol production from biodiesel-derived crude glycerol under non-strictly anaerobic conditions[J], PROCESS BIOCHEMISTRY, 2019, 83:27-34.
[6] Sun, Yaqin, Xu, ZhenZhen, Zheng,Yafeng, Zhou, Jinjie, Xiu, Zhilong. Efficient production of lactic acid from sugarcane molasses by a newly microbial consortium CEE-DL15[J], PROCESS BIOCHEMISTRY, 2019, 81:132-138.
[7] Hou, Mengyang, Sun, Jiao, Hu, Wenzhong, Wang, Aosheng, Xiu, Zhilong, Shi, Yusheng, Hao, Kexin, Sun, Xingsheng, Cao, Duo, Lu, Ruishan. Ultrasound-Assisted Extraction of Total Flavonoids from Pteris cretica L.: Process Optimization, HPLC Analysis, and Evaluation of Antioxidant Activity[J], ANTIOXIDANTS, 2019, 8(10)
[8] Yuan, Jinlong, Xie, Jun, Liu, Chongyang, Teo, Kok Lay, Huang, Ming, Fan, Houming, Feng, Enmin, Xiu, Zhilong. Robust optimization for a nonlinear switched time-delay system with noisy output measurements using hybrid optimization algorithm[J], JOURNAL OF THE FRANKLIN INSTITUTE-ENGINEERING AND APPLIED MATHEMATICS, 2019, 356(16): 9730-9762
[9] Pan, Duo-Tao, Wang, Xu-Dong, Shi, Hong-Yan, Yuan, De-Cheng, Xiu, Zhi-Long. Ensemble optimization of microbial conversion of glycerol into 1, 3-propanediol by Klebsiella pneumonia[J], JOURNAL OF BIOTECHNOLOGY, 2019, 301: 68-78
[10] Dong, Yue-Sheng, Yang, Fan, Liu, Wen-Cai, Wang, Xu-Dong, Xing, Yan, Xiu, Zhi-Long. Separation and purification of wortmannilactone analogues by three-liquid-phase salting-out extraction coupled with column chromatography[J], SEPARATION AND PURIFICATION TECHNOLOGY, 2019, 224: 397-404
[11] Li, Zhen, Yan, Ling, Zhou, Jinjie, Wang, Xiaoli, Sun, Yaqin, Xiu, Zhi-Long. Two-step salting-out extraction of 1,3-propanediol, butyric acid and acetic acid from fermentation broths[J], SEPARATION AND PURIFICATION TECHNOLOGY, 2019, 209: 246-253
[12] Yuan, Yuyu, Qu, Kunli, Tan, Demeng, Li, Xiaoyu, Wang, Lili, Cong, Cong, Xiu, Zhilong, Xu, Yongping. Isolation and characterization of a bacteriophage and its potential to disrupt multi-drug resistant Pseudomonas aeruginosa biofilms[J], Microbial Pathogenesis, 2019, 128:329-336
[13] Dai, Jianying, Wang, Zhuangfei, Xiu, Zhi-Long. High production of optically pure (3R)-acetoin by a newly isolated marine strain of Bacillus subtilis CGMCC 13141[J], BIOPROCESS AND BIOSYSTEMS ENGINEERING, 2019, 42(3): 475-483
[14] Pan, Duo-Tao, Wang, Xu-Dong, Shi, Hong-Yan, Yuan, De-Cheng, Xiu, Zhi-Long. Dynamic flux balance analysis for microbial conversion of glycerol into 1,3-propanediol byKlebsiella pneumoniae[J], BIOPROCESS AND BIOSYSTEMS ENGINEERING, 2018, 41(12): 1793-1805
[15] Wang, Xu-Dong, Wei, Ning-Ning, Wang, Shu-Chang, Yuan, Heng-Li, Zhang, Feng-Ying, Xiu, Zhi-Long. Kinetic Optimization and Scale-Up of Site-Specific Thiol-PEGylation of Loxenatide from Laboratory to Pilot Scale[J], INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, 2018, 57(44): 14915-14925
[16] Sun, Yaqin, Zhang, Shuangshuang, Zhang, Xingxing, Zheng, Yafeng, Xiu, Zhilong. Ionic liquid-based sugaring-out and salting-out extraction of succinic acid[J], SEPARATION AND PURIFICATION TECHNOLOGY, 2018, 204: 133-140
[17] Zhou, Jin-Jie, Shen, Jun-Tao, Wang, Xiao-Li, Sun, Ya-Qin, Xiu, Zhi-Long. Stability and oscillatory behavior of microbial consortium in continuous conversion of crude glycerol to 1,3-propanediol[J], APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2018, 102(19): 8291-8305
[18] Dou, Shaohua, Chi, Naiyu, Zhou, Xinshang, Zhang, Qingfang, Pang, Fei, Xiu, Zhilong. Molecular cloning, expression, and biochemical characterization of a novel cold-active alpha-amylase fromBacillussp dsh19-1[J], EXTREMOPHILES, 2018, 22(5): 739-749
[19] Wang, Xudong, Wang, Shuchang, Pan, Duotao, Qin, Kairong, Yuan, Hengli, Zhang, Fengying, Sun, Yundong, Xiu, Zhilong. Combined optimization of N-terminal site-specific PEGylation of recombinant hirudin using response surface methodology and kinetic analysis[J], ENGINEERING IN LIFE SCIENCES, 2018, 18(8): 611-621
[20] Dai, Jianying, Wang, Hui, Li, Yan, Xiu, Zhi-Long. Imidazolium ionic liquids-based salting-out extraction of 2,3-butanediol from fermentation broths[J], PROCESS BIOCHEMISTRY, 2018, 71: 175-181
[21] Sun, Ya-Qin, Shen, Jun-Tao, Yan, Ling, Zhou, Jin-Jie, Jiang, Li-Li, Chen, Yang, Yuan, Jin-Long, Feng, Enmin, Xiu, Zhi-Long. Advances in bioconversion of glycerol to 1,3-propanediol: Prospects and challenges[J], PROCESS BIOCHEMISTRY, 2018, 71: 134-146
[22] Zhang, Bowei, Xiu, Zhilong, Ma, Baiping, Yu, Liyan, Dong, Yuesheng, Sun, Wenlong, Yu, Na, Sun, Jin, Yu, Xiaoxia, Li, Xia, Xing, Yan, Yan, Dong, Ding, Qianzhi. Anti-diabetic effect of baicalein is associated with the modulation of gut microbiota in streptozotocin and high-fat-diet induced diabetic rats[J], JOURNAL OF FUNCTIONAL FOODS, 2018, 46: 256-267
[23] Yan, Ling, Sun, Ya-Qin, Wang, Xu-Dong, Fu, Hong-Xin, Mu, Ying, Xiu, Zhi-Long. Partition behavior of monocarboxylic acids in salting-out extraction systems of monohydric alcohols and dipotassium phosphate[J], SEPARATION AND PURIFICATION TECHNOLOGY, 2018, 199: 351-358
[24] 胡春燕, 王旭东, 秦超, 董悦生, 修志龙. 克拉维酸钾一步结晶工艺优化[J], 高校化学工程学报, 2018, 32(4): 910-917
[25] Zhao, Shan, Yang, Fan, Liu, Yang, Sun, Dongsheng, Xiu, Zhilong, Ma Xiaojun, Zhang, Ying, Sun, Guangwei. Study of chemical characteristics, gelation properties and biological application of calcium pectate prepared using apple or citrus pectin.[J], International journal of biological macromolecules, 2018, 109: 180-187
[26] Xing, Yan, Chen, Chaonan, Sun, Wenlong, Zhang, Bowei, Sang, Yuanbin, Xiu, Zhilong, Dong, Yuesheng. An environment-friendly approach to isolate and purify glucan from spent cells of recombinantPichia pastorisand the bioactivity characterization of the purified glucan[J], ENGINEERING IN LIFE SCIENCES, 2018, 18(4): 227-235
[27] 李敬达, 余承洁, 王仁军, 付常振, 修志龙, 刘庆平. 慢病毒介导CD36基因沉默细胞株的构建及其对caveolin-1蛋白表达的影响[J],生物工程学报, 2018, 34(01): 110-121
[28] Zhao, Shan, Zhang, Ying, Liu, Yang, Yang, Fan, Xiu, Zhilong, Ma, Xiaojun, Sun, Guangwei. Preparation and optimization of calcium pectate beads for cell encapsulation[J], JOURNAL OF APPLIED POLYMER SCIENCE, 2018, 135(2)
[29] 鄢凌, 傅宏鑫, 王旭东, 孙亚琴, 戴建英, 修志龙. 生物基有机酸提取分离技术研究进展[J], 过程工程学报, 2018, 18(1):1-10
[30] Zhen, Weilong, Li, Jingda, Wang, Xudong, Yuan, Yanping, Zhang, Jianing, Xiu, Zhilon. Effects of Antarctic krill docosahexaenoic acid on MCF-7 cell migration and invasion induced by the interaction of CD95 and caveolin-1[J], Life Science, 2018, 192(2): 270-277
[31] Zheng, Weilong, Li, Jingda, Wang, Xudong, Yuan, Yanping, Zhang, Jianing, Xiu, Zhilong. Effects of Antarctic krill docosahexaenoic acid on MCF-7 cell migration and invasion induced by the interaction of CD95 with caveolin-1[J], LIFE SCIENCES, 2018, 192:270-277
[32] Sun Wenlong, Zhang Bowei, Yu Xiaoxia, Zhuang Chunlin, Li Xia, Sun Jin, Xing Yan, Xiu Zhilong, Dong Yuesheng. Oroxin A from Oroxylum indicum prevents the progression from prediabetes to diabetes in streptozotocin and high-fat diet induced mice.[J], Phytomedicine: international journal of phytotherapy and phytopharmacology, 2018, 38: 24-34
[33] Liu, Wen-Cai, Dong, Yue-Sheng, Ren, Yi-Xin, Hao, Ai-Yu, Yu, Sun, Shi, Xuan, Zhang, Xue-Qiang, Xing, Yan, Xiu, Zhi-Long, Cui, Yu. The activities of wortmannilactones against helminth electron transport chain enzymes, structure-activity relationships, and the effect onTrichinella spiralisinfected mice.[J], The Journal of antibiotics, 2018, 71(8): 731-740
[34] Jiang, Lili, Dai, Jianying, Sun, Yaqin, Xiu, Zhilong. The effects of ionic liquid 1-ethyl-3-methylimidazolium trifluoromethanesulfonate on the production of 1,3-propanediol from crude glycerol by microbial consortium.[J], Bioprocess and biosystems engineering, 2018, 41(8): 1079-1088
[35] Wang, Xudong, Lv, Li, Qin, Kairong, Yuan, Hengli, Zhang, Fengying, Chen, Guohui, Xiu, Zhilong. Effects of linear and branched polyethylene glycol on PEGylation of recombinant hirudin: Reaction kinetics and in vitro and in vivo bioactivities[J], PROCESS BIOCHEMISTRY, 2017, 63: 154-162
[36] Zhang, Bo-wei, Xing, Yan, Wen, Chen, Yu, Xiao-xia, Sun, Wen-long, Xiu, Zhi-long, Dong, Yue-sheng. Pentacyclic triterpenes as alpha-glucosidase and alpha-amylase inhibitors: Structure-activity relationships and the synergism with acarbose[J], BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2017, 27(22): 5065-5070
[37] Sun, Wenlong, Sun, Jin, Zhang, Bowei, Xing, Yan,Yu, Xiaoxia, Li, Xia, Xiu, Zhilong, Dong, Yuesheng. Baicalein improves insulin resistance via regulating SOCS3 and enhances the effect of acarbose on diabetes prevention[J], JOURNAL OF FUNCTIONAL FOODS, 2017, 37: 339-353
[38] Zhang, Bo-wei, Li, Xia, Sun, Wen-long, Xing, Yan, Xiu, Zhi-long, Zhuang, Chun-lin, Dong, Yue-sheng. Dietary Flavonoids and Acarbose Synergistically Inhibit alpha-Glucosidase and Lower Postprandial Blood Glucose[J], JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2017, 65(38): 8319-8330
[39] 沈俊涛,修志龙.工业微生物的噬菌体感染与防治策略[J],生物工程学报, 2017, 33(12): 1901-1912
[40] Dai, Jianying, Guan, Wentian, Ma, Linhui, Xiu, Zhilong. Salting-out extraction of acetoin from fermentation broth using ethyl acetate and K2HPO4[J],SEPARATION AND PURIFICATION TECHNOLOGY, 2017, 184: 275-279
[41] 张超蕾,周瑾洁,姜莉莉,王晓丽,朱甲名,权春善,修志龙.微生物组学及其应用研究进展[J],微生物学杂志, 2017, 37(4): 74-81
[42] Zhou, Jin-Jie, Shen, Jun-Tao, Jiang, Li-Li, Sun, Ya-Qin, Mu, Ying, Xiu, Zhi-Long. Selection and characterization of an anaerobic microbial consortium with high adaptation to crude glycerol for 1,3-propanediol production[J], APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2017, 101(15): 5985-5996
[43] Jiang Li-Li, Zhou Jin-Jie, Quan Chun-Shan, Xiu Zhi-Long. Advances in industrial microbiome based on microbial consortium for biorefinery.[J], Bioresources and bioprocessing, 2017, 4(1):11
[44] Zhang, Bo-wei, Sang, Yuan-bin, Sun, Wen-long,Y u, He-shui, Ma, Bai-ping, Xiu, Zhi-long, Dong, Yue-sheng. Combination of flavonoids from Oroxylum indicum seed extracts and acarbose improves the inhibition of postprandial blood glucose: In vivo and in vitro study[J], BIOMEDICINE & PHARMACOTHERAPY, 2017, 91: 890-898
[45]杨琦,蒋志刚,冯恩民,尹洪超,修志龙.以函数为参量的间歇发酵非线性动力系统及其辨识[J],运筹学学报, 2017, 21(2): 46-56
[46] Fu, Hongxin, Wang, Xudong, Sun, Yaqin, Yan, Ling, Shen, Juntao, Wang, Jufang, Yang, Shang-Tian, Xiu, Zhilong. Effects of salting-out and salting-out extraction on the separation of butyric acid[J], SEPARATION AND PURIFICATION TECHNOLOGY, 2017, 180: 44-50
[47] Jiang, LiLi, Liu, HuiFang, Mu, Ying, Sun, YaQin, Xiu, ZhiLong. High tolerance to glycerol and high production of 1,3-propanediol in batch fermentations by microbial consortium from marine sludge[J], ENGINEERING IN LIFE SCIENCES, 2017, 17(6): 635-644
[48] Yuan, Jinlong, Zhang, Yuduo, Ye, Jianxiong, Xie, Jun, Teo, Kok Lay, Zhu, Xi, Feng, Enmin, Yin, Hongchao, Xiu, Zhilong. Robust parameter identification using parallel global optimization for a batch nonlinear enzyme-catalytic time-delayed process presenting metabolic discontinuities[J], APPLIED MATHEMATICAL MODELLING, 2017, 46: 554-571
[49] Li, Jingda, Xiu, Zhilong, Wang, Renjun, Yu, Chengjie, Chi, Yan, Qin, Jianzhong, Fu, Changzhen, Matsuura, Eiji, Liu, Qingping. The lipid moiety 7-ketocholesteryl-9-carboxynonanoate mediates binding interaction of oxLDL to LOX-1 and upregulates ABCA1 expression through PPAR gamma[J], LIFE SCIENCES, 2017, 177: 27-40
[50] Li, Na, Sun, Guangwei, Wang, Shujun, Wang, Yu, Xiu, Zhilong, Sun, Dongsheng, Guo, Xin, Zhang, Ying, Ma, Xiaojun. Engineering islet for improved performance by optimized reaggregation in alginate gel beads[J], BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 2017, 64(3): 400-405
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修志龙教授 博士 教授
Prof. Xiu Zhi-Long
E-mail:zhlxiu@
董悦生 博士 副教授
Assoc. Prof. Dong Yue-Sheng
E-mail: yshdong@
戴建英 博士 副教授
Assoc. Prof. Dai Jian-Ying
E-mail: jydai@
孙亚琴 博士 副教授
Assoc. Prof. Sun Ya-Qin
E-mail: sunyaqin@