生物医学吸附材料研究室-大连理工大学生物工程学院（新）

团队简介

课题组的研究工作主要聚焦于发展生物分离分析领域的新材料和新技术，及其在生物医学、环境污染物修复等方面的应用。基础研究重点发展创新的生物分子识别技术和血液相容性生物医学材料；应用研究侧重于治疗疑难病症的特异性血液净化吸附材料。

近年来承担“十三五”国家重点研发项目、“863”项目、国家自然科学基金项目及各类省市等项目近20项，科研经费总额达到2000余万元。发表学术论文60余篇，授权发明专利21项，其中发明的治疗重症肌无力等自身免疫性疾病“仿生蛋白A吸附柱”已完成产业化中试，进入临床试验；治疗脓毒血症的内毒素吸附柱、治疗肾衰竭的中分子精准血液净化吸附柱已完成小试，进入中试放大转化阶段。

The group’s work is at the interface of biotechnology and materials science. A major focus is the development of novel materials and technologies for the separation and analysis of biomolecules, including their applications in the biomedical and environmental fields. While conducting basic research such as the development of innovative biomolecular recognition techniques and blood-compatible biomedical materials, we also have strong interest in the bench-to-bedside research, especially the industrialization of specific blood purification adsorbents for the treatment of life-threatening diseases.

Prof. Jia has led nearly 20 key government-sponsored research projects, received funds exceeding 20 millions, published more than 60 peer reviewed journal articlesand been granted 21 patents.

There are nearly 50 members in the group, including 1 professor, 5 associate professors, 1 senior engineer, more than 30 Ph.D and master students, as well as collaborators both at home and abroad.

代表性成果

生物医学吸附材料研究室科研团队依托辽宁省分子识别与成像重点实验室和大连市血液净化吸附材料工程实验室，搭建了从精准血液净化材料的分子设计到材料构建及有效性和安全性评价的研发平台。

产业化平台主要依托大连市生物医学材料转化中心，该基地占地面积为7000平米，目前已完成投资近9000万元，产业化设备590台（套），具有一流的公用工程配套和符合GMP要求净化车间，能够快速实现研究成果转化。已发展和建立了中大分子毒素精准血液净化吸附剂集成化技术、蛋白结合疏水性毒素水溶性分子吸附剂规模化生产技术，开发具有原创性的精准安全血液净化新产品；针对癌症诊疗的迫切需求，开发基于循环肿瘤细胞捕获的创新技术。形成一批国际领先水平的新型血液净化吸附材料生产新技术、新工艺，获得一批国际国内发明专利，建立了血液净化吸附材料从科研向产品转化的产业化示范。

专利情况

1	一种蛋白质功能化冰胶材料、其制备方法和用途
2	一种连续流固定化甲酰甘氨酸生成酶反应器及其使用方法与应用
3	一种花粉结构微粒及其制备方法和用途
4	一种抗体复合物的制备方法
5	一种疏水层析介质、制备方法及其应用
6	细胞复刻表面及其应用
7	一种模块化小室及其对血液中稀有细胞高效捕获的应用
8	用于循环肿瘤细胞捕获的多酚涂层
9	一种用于纯化并定向固定化组氨酸标签蛋白的方法和应用
10	一种层析介质及其制备方法和应用
11	利用多酚类化合物在材料表面实现血小板图案化的方法
12	一种抗人淀粉样β肽的纳米抗体及其应用
13	一种重组蛋白A亲和配基的定点固定化方法
14	抗人β2微球蛋白的单域抗体及其制备方法和用途
15	一种抗人甲状旁腺激素的纳米抗体及其筛选方法和应用
16	环糊精修饰磁性纳米吸附剂的制备方法及其在血液透析吸附系统中的应用
17	用于清除类风湿因子的血液净化材料及其制备方法
18	一种特异性清除甲状旁腺激素的血液净化吸附剂的制备方法
19	利用重组毕赤酵母生产蛋白A的方法
20	一种重组人IgE受体蛋白的制备方法及应用
21	一种对抗体具有广谱吸附能力的融合蛋白制备方法及应用
22	一种以分子簇为功能基的内毒素吸附剂及其制备方法
23	一种生产重组蛋白A的方法
24	BLOOD PURIFICATION ADSORBENT USEFUL FOR ELIMINATING ANTIBODY
25	偶联环糊精的水溶性多聚物吸附材料及其应用
26	一种用于抗体清除的血液净化吸附剂及其制备方法
27	用于治疗内毒素血症的内毒素吸附材料
28	一种革兰氏阴性菌微胶囊及其制备方法
29	一种用于治疗高脂蛋白血症的低密度脂蛋白吸附材料
30	用于治疗高胆红素血症的胆红素吸附材料
31	一株降解多氯联苯的兼性厌氧菌及获得方法
32	用于治疗高胆红素血症的免疫吸附材料
33	用于血液净化的蛋白A免疫吸附材料的合成方法
34	用于从血浆中去除致病抗体及其复合物的蛋白免疫吸附介质的合成方法
35	一种高效亲和膜色谱介质及其合成方法
36	以纤维素为基质的亲和膜介质的合成方法
37	径向色谱柱用金属螯合亲和膜色谱介质的合成方法

论文情况

1. Huang, Chundong; Ren, Jun; Ji, Fangling; Muyldermans, Serge; Jia, Lingyun. Nanobody-Based high-performance immunosorbent for selective beta 2-microglobulin purification from blood. DOI:10.1016/j.actbio.2020.02.028
2. He Sun, Lulu Han, Liwei Yang, Yan Yang, Wenning Jiang, Ting Xu, Lingyun Jia. Modular Chamber Assembled with Cell-Replicated Surface for Capture of Cancer Cells. ACS Biomater. Sci. Eng. 2019, 5, 5, 2647-2656
3. Zang, B; Ren, J; Li, D; Huang, CD ; Ma, H; Peng, Q; Ji, FL; Han, LL; Jia, LY. Freezing-assisted synthesis of covalent C-C inked bivalent and bispecific nanobodies. Organic & Biomolecular Chemistry. 2019,17(2): 257-263 WOS:000454946700026
4. Xu, L; Wang, R; Cao, HY ; Xu, T; Han, LL; Huang, CD; Jia, LY. A facile method to oriented immobilization of His-tagged BirA on Co3+ -NTA agarose beads. Enzyme and Microbial Technology.2019,120: 36-42 DOI: 10.1016/j.enzmictec.
5. Ji, FL ; Feng, YB ; Li, MY ; Long, FD ; Yang, YL ; Wang, TQ ; Wang, JY ; Bao, YM; Xue, S. Structure and catalytic mechanistic insight into Enterobacter aerogenes acetolactate decarboxylase. ENZYME AND MICROBIAL TECHNOLOGY. 2019,126: 9-17, DOI: 10.1016/j.enzmictec.
6. Zhou, YM; Gao, XR. Characterization of Biofilm Formed by Phenanthrene-Degrading Bacteria on Rice Root Surfaces for Reduction of PAH Contamination in Rice. Int. J. Environ. Res. Public Health 2019, 16, 2002; doi:10.3390/ijerph16112002
7. Yang,Liwei; Sun,He; Jiang,Wenning; Xu,Ting; Song,Bing; Peng,Ruilian; Han,Lulu; Jia, Lingyun. A Chemical Method for Specific Capture of Circulating Tumor Cells Using Label-Free Polyphenol-Functionalized Films. CHEMISTRY OF MATERIALS, 2018, 30(13):4372-4382. WOS:000438653300022
8. Li, Jingyu; Han, Lulu; Liu, Shuxin; He, Sai; Cao, Yaming; Xie, Jian; Jia, Lingyun.* Removal of indoxyl sulfate by water-soluble poly-cyclodextrins in dialysis. Colloids and surfaces. B, Biointerfaces. 2018，164:406-413.
9. Zang,Berlin; Jia,Lingyun; Ren,Jun; Peng,Qiang. Facile preparation of covalent C-to-C linked nanobody fusions by freezing. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2018,255:437. WOS:000435537701550
10. Yang,Liwei; Han,Lulu; Jia,Lingyun. Galloyl groups-regulated fibrinogen conformation: Understanding antiplatelet adhesion on tannic acid coating. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2018, 255:495. 0065-7727, WOS:000435539906477；CPCI-S：000435539906477
11. Li, Jingyu; Han, Lulu; Liu, Shuxin; He, Sai; Cao, Yaming; Xie, Jian; Jia, Lingyun。 Removal of indoxyl sulfate by water-soluble poly-cyclodextrins in dialysis，Colloids and surfaces. B, Biointerfaces, .2018,164:406-413. WOS:000429762100047
12. LiweiYang, LuluHan, QiLiu, YigeXu, Lingyun,Jia*, Galloyl groups-regulated fibrinogen conformation: Understanding antiplatelet adhesion on tannic acid coating. Acta Biomaterialia, 64 (2017) 187–199. WOS: 000416498200017
13. Han, L., Liu, Q.,Yang, L.,Ye, T.,He, Z.,Jia, L. *，Facile Oriented Immobilization of Histidine-Tagged Proteins on Nonfouling Cobalt Polyphenolic Self-Assembly Surfaces. ACS Biomaterials Science and Engineering, 2017,3(12) :3328-3337. WOS:000418110600029
14. Xu L, Song X, Jia L*. A camelid nanobody against EGFR was easily obtained through refolding of inclusion body expressed in Escherichia coli. Biotechnol Appl Biochem. 2017,64(6):895-901. WOS:000418111300014
15. Ming Gao, Jun Ren∗, Kaikai Tian, Lingyun Jia*.Characterization of non-specific protein adsorption induced by triazole groups on the chromatography media using Cu (I)-catalyzed alkyne-azide cycloaddition reaction for ligand immobilization. Journal of Chromatography A, 2016,1476:63–68.
16. Liwei Yang, Lulu Han and Lingyun Jia*, A novel platelet repellent polyphenolic surface and its micropattern for platelet adhesion detection. ACS Applied Materials & Interfaces, 2016, 8(40), 26570-26577. WOS:000385469000016
17. Jun Ren, Kaikai Tian, Lingyun Jia*, Xiuyou Han and Mingshan Zhao, Rapid covalent immobilization of proteins by phenol-based photochemical cross-linking. Bioconjugate Chemistry, 2016, 27(10), 2266-2270. WOS:000385992000006
18. Ren BP, Jiang BB, Hu RD, Zhang MZ, Chen H, Ma J, Sun Y, Jia LY, Zheng J, HP-beta-cyclodextrin as an inhibitor of amyloid-beta aggregation and toxicity. PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2016,18(30):20476-20485. WOS:000381428600052
19. Ren BP, Zhang MZ, Gao HP, Zheng J, Jia LY*, Atomic elucidation of the cyclodextrin effects on DDT solubility and biodegradation. PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2016,18(26):17380-17388
20.Conggang Wang, Fang Cheng, Li Xu, Lingyun Jia*, HSA targets multiple A beta 42 species and inhibits the seeding–mediated aggregation and cytotoxicity of A beta 42 aggregates. RSC ADVANCES: 2016 (75) , 71165-71175