低碳代谢设计与重编程研究室常年招聘代谢工程与合成生物学、系统生物学、生物信息学、微生物学、蛋白质工程、发酵工程等研究方向的博士后，招收生物工程、生物化工专业博士生及硕士生，生物与医药、生物学硕士生。

本研究室近期招聘副教授（1名），助理教授（1名），欢迎有科研热情、对学术交叉感兴趣的优秀青年加盟低碳代谢设计与重编程研究室！

学术带头人：

吴辉，江苏苏州，工学博士，教授，博导。入选国家级高层次青年人才，教育部“霍英东青年教师基金” 获得者。中国生物工程学会青年工作委员会委员，中国生物工程学会一碳生物技术专委会委员，上海市生物工程学会一碳生物技术专委会副主任委员，上海市生物工程学会合成生物学委员会委员，教育部智能生物制造重点实验室固定人员。荣获宝钢优秀教师奖、侯德榜化工技术奖青年奖、“东富龙”生物工程优秀青年科学家奖。获上海市教学成果奖一等奖（排名第五）、中国石油与化工教育教学优秀成果一等奖（排名第一），华东理工大学教学成果一等奖（排名第一）。2004年毕业于南京工业大学生物工程系，获工学学士学位。2009年毕业于华东理工大学发酵工程专业，获工学博士学位。同年进入中国科学院上海生命科学研究院植物生理生态研究所从事博士后研究工作。2011年来到誉有“南方的哈佛”之称的美国莱斯大学生物工程系从事博士后研究工作。2014年9月引进华东理工大学生物工程学院。2024年1月入职大连理工大学生物工程学院。作为负责人主持国家自然科学基金面上项目（两项）、青年项目、国家重点研发计划课题、国家重点研发计划子课题（两项）、教育部“霍英东青年教师基金” 、上海市自然科学基金及企业技术开发等项目。作为负责人主持上海市教改项目两项：《微生物学（全英文）》入选上海市高校示范性全英语课程建设项目和《微生物学》入选上海市一流本科课程。近年来在Metabolic Engineering，Biotechnology Advances，ACS Synthetic Biology，Biotechnology and Bioengineering，Bioresource Technology，ACS Sustainable Chemistry & Engineering等本领域重点期刊上发表SCI收录论文60余篇。已授权中国发明专利11项；已授权美国发明专利4项。指导研究生多次获得校外奖学金、学术会议最佳墙报奖和创新竞赛等奖项；共同指导本科生多次获得国际遗传工程机器大赛(iGEM)竞赛金奖，指导 “大学生创新创业训练计划”国家级、上海市级和校级等项目，多次获竞赛奖项。

研究方向：

1．微生物低碳代谢途径理性设计和调控

运用代谢途径工程和合成生物学的最新技术和方法，结合生物相容性材料工程，通过智能机器学习和实验室自动化筛选策略，设计及重构微生物代谢途径，构建以可再生生物质资源（如乙酸、乙醇、甲酸、CO₂等）为原料，利用多元化能量来源（化能和电能等）的新型高效微生物细胞工厂，合成多种生物基化学品。

2．基于 “代谢晶体管”的代谢重编程

在微生物代谢调控合成生物学研究中，“代谢晶体管”控制模式借鉴电子元器件“晶体管”的放大控制模式，通过精细调节细胞中微量关键物质的浓度，进而对整个细胞进行代谢重编程，实现目标产物的“智能”代谢控制。

主持和参加的科研项目：

1．主持国家自然科学基金面上基金 2023.01 - 2026.12

2．主持国家重点研发计划课题 2022.11 - 2026.10

3．主持国家重点研发计划子课题 2021.12 - 2024.11

4．主持国家自然科学基金面上基金 2018.01 - 2021.12

5．主持国家重点研发计划子课题 2017.07 - 2021.06

6．主持霍英东教育基金会第十六届高等院校青年教师基金 2018.3-2021.3

7．主持上海市自然科学基金 2019.6-2022.6

8．主持国家自然科学基金青年科学基金 2015.1-2017.12

1．Ka-Yiu San, Xian Zhang, Hui Wu, Dan Wang. KAS III-free fatty acid synthesis. US Patent Number: 10851393B2. 授权.

2．Ka-Yiu San, George N. Bennett, Hui Wu. Metabolic transistors in bacteria. US Patent Number: 10,011,839 B2. 授权.

3．Ka-Yiu San, Xixian Xie, Leepika Tuli, Hui Wu. Hydroxy- and dicarboxylic-fat synthesis by microbes. US patent Number: 9,487,804 B2. 授权.

4．Ka-Yiu San, George N. Bennett, Hui Wu. Metabolic transistors in bacteria. US Patent Number: 9,441,253 B2. 授权.

5.  罗远婵，吴辉. 一种高耐镉的生防解淀粉芽孢杆菌及其应用. 中国发明专利. 专利申请号：202110666584. 授权.

6.  范建华，吴辉，周冬青. 一种转基因微藻及其在调控代谢产物合成中的应用. 中国发明专利，专利号：ZL202110303222.0. 授权.

7．吴辉，陆静娴，李志敏，叶勤. 一种提高大肠杆菌合成的聚（3-羟基丁酸-co-乳酸）中乳酸组分含量的方法.中国发明专利. 专利号：ZL201810246568.X. 授权.

8．吴辉，杨昊，李志敏，黄兵，叶勤. 利用乙酸生产丙酮或异丙醇的代谢工程大肠杆菌菌株的构建方法与应用. 中国发明专利. 专利号：ZL201710978174.9. 授权.

9．吴辉，黄兵，李志敏，杨昊，叶勤. 利用乙酸生产羟基丙酸的代谢工程大肠杆菌菌株的构建方法与应用. 中国发明专利. 专利号：ZL201710969403.0. 授权.

10．叶勤，李晴，李志敏，吴辉. 利用重组大肠杆菌以甘油为碳源发酵生产琥珀酸的方法. 中国发明专利. 专利号：ZL201610305322.6. 授权.

11．李志敏, 黄兵, 吴辉, 房国辰, 叶勤.一种利用造纸废液生物合成丁二酸的方法. 中国发明专利. 专利号: ZL201711142577.6. 授权

12．李志敏, 李运杰, 吴辉, 叶勤. 利用乙酸生产丁二酸的代谢工程大肠杆菌菌株构建方法和应用. 中国发明专利. 专利号：ZL201410599042.1. 授权.

13．孙周通，刘映淼，孙兵兵，王祎，吴辉，杨俊杰等. L-色氨酸基因工程菌,其构建方法以及使用其发酵生产L-色氨酸的方法. 中国发明专利. 专利号：ZL201010598350. 授权.

14．叶勤，李志敏，吴辉. 利用四碳二元酸发酵固定二氧化碳的方法. 中国发明专利. 专利号：ZL200810207564. 授权.

15．吴辉，李志敏，叶勤. 大肠杆菌生产琥珀酸的方法. 中国发明专利. 专利号：ZL200710135684. 授权.

1. Ju Wu,^# Xuan Gong,^# Xinye Sun, Pengye Guo, Yuanchan Luo, Hui Wu*.  Fine-tuning biosynthesis of biodegradable  poly(3-hydroxybutyrate-co-lactate) with different ratios of D-lactate  from xylose by engineered Escherichia coli. ACS Sustainable Chemistry & Engineering. 2023. 11:15043–15051. (JCR Q1)

2. Mingcheng Xu^#, Yuanchan Luo^#, Xiaozhen Liu^#, Peng Fei, Juefeng Lu, Yuying Wang, Hui Wu*. Production of 3-hydroxypropionic acid from syngas-derived acetic acid by engineered Vibrio natriegens combined with adaptive laboratory evolution. ACS Sustainable Chemistry & Engineering. 2023. 11:4125–4132. (JCR Q1)

3. Yuying Wang^#, Juefeng Lu^#, Mingcheng Xu, Chen Qian, Fei Zhao, Yuanchan Luo*, Hui Wu*. Efficient biosynthesis of isopropanol from ethanol by metabolically engineered Escherichia coli. ACS Sustainable Chemistry & Engineering. 2022.10: 13857-13864. (JCR Q1)

4. Juefeng Lu, Yuying Wang, Mingcheng Xu, Yuanchan Luo, Hui Wu*. Efficient biosynthesis of 3-hydroxypropionic acid from ethanol in metabolically engineered Escherichia coli. Bioresource Technology. 2022, 363:127907. (JCR Q1)

5. Yuanchan Luo, Lei Chen, Zhibo Lu, Weijian Zhang, Wentong Liu, Yuwei Chen, Xinran Wang, Wei Du, Jinyan Luo*, Hui Wu*. Genome sequencing of biocontrol strain Bacillus amyloliquefaciens Bam1 and further analysis of its heavy metal resistance mechanism. Bioresources and Bioprocessing. 2022, 9:74. (JCR Q2)

6. Dongqing Zhou, Yeling Luo, Yingqiu Zhang, Chenni Zhao, Hui Wu*, Jianhua Fan*. Perturbing electron carriers of green algae Chlamydomonas reinhardtii for efficient physiological and metabolic regulation. Food Bioengineering. 2022, 1: 148-158.

7. Ju Wu^#, Xiangju Wei^#, Pengye Guo, Aiyong He, Jiaxing Xu, Mingjie Jin, Yanjun Zhang, Hui Wu*. Efficient P(3HB-co-LA) production from corn stover hydrolysate by metabolically engineered Escherichia coli. Bioresource Technology. 2021, 341:125873. (JCR Q1)

8. Peng Fei^#, Yuanchan Luo^#, Ningyu Lai, Hui Wu*. Biosynthesis of (R)-3-hydroxybutyric acid from syngas-derived acetate in engineered Escherichia coli. Bioresource Technology. 2021, 336:125323. (JCR Q1)

9. Ningyu Lai^#, Yuanchan Luo^#, Peng Fei, Peng Hu, Hui Wu*. One stone two birds: biosynthesis of 3-hydroxypropionic acid from CO₂ and syngas-derived acetate in Escherichia coli. Synthetic and Systems Biotechnology. 2021, 6: 144-152. (JCR Q2)

10. Jianhua Fan, Dongqing Zhou, Cheng Chen, Ju Wu, Hui Wu*. Reprogramming the metabolism of Synechocystis PCC 6803 by regulating the plastoquinone biosynthesis. Synthetic and Systems Biotechnology. 2021, 6: 351-359. (JCR Q2)

11. Pengye Guo^#, Yuanchan Luo^#, Ju Wu, Hui Wu*. Recent advances in the microbial synthesis of lactate-based copolymer. Bioresources and Bioprocessing. 2021, 8: 106. (JCR Q2)

12. Na Zhang^♯, Hui Wu^♯, Yingzhi Liang^♯,  Jianming Ye, Huan Zhang, Yuqing Miao, Yane Luo*, Haiming Fan , Tianli  Yue. Design and preparation of “corn-like” SPIONs@DFK-SBP-M13 assembly  for improvement of effective internalization. International Journal of Nanomedicine. 2021. 16:7091-7102. (JCR Q1).

13. Xiangju Wei, Ju Wu, Pengye Guo, Shengmin Zhou, Hui Wu*. Effect of short-chain thioesterase deficiency on p(3HB-co-LA) biosynthesis in Escherichia coli. Chinese Journal of Biotechnology. 2021. 37(1):196-206.

14. Guohui Li, Dixuan Huang, Xue Sui, Shiyun Li, Bing Huang, Xiaojuan Zhang*, Hui Wu*, Yu Deng*. Advances in microbial production of medium-chain dicarboxylic acids for nylon materials. Reaction Chemistry & Engineering. 2020. 5:221-238 (JCR Q2)

15. Hao Yang, Can Zhang, Ningyu Lai, Bing Huang, Peng Fei, Dawei Ding, Peng Hu, Yang Gu, Hui Wu*. Efficient isopropanol biosynthesis by engineered Escherichia coli using biologically produced acetate from syngas fermentation. Bioresource Technology. 2020. 296:122337. (JCR Q1)

16. Qiaofei He, George N. Bennett, Ka-Yiu San, Hui Wu*. Biosynthesis of medium-chain ω-hydroxy fatty acids by AlkBGT of Pseudomonas putida GPo1 with native FadL in engineered Escherichia coli. Frontiers in Bioengineering and Biotechnology. 2019. 7:273. (JCR Q1)

17. Hao Yang^#, Bing Huang^#, Ningyu Lai, Yang Gu, Zhimin Li, Qin Ye, Hui Wu*. Metabolic engineering of Escherichia coli carrying the hybrid acetone-biosynthesis pathway for efficient acetone biosynthesis from acetate. Microbial Cell Factories. 2019. 18:6. (JCR Q1)

18. Jingxian Lu, Zhimin Li, Qin Ye, Hui Wu*. Effect of reducing the activity of respiratory chain on biosynthesis of poly(3-hydroxybutyrate-co-lactate) in Escherichia coli. Chinese Journal of Biotechnology. 2019. 35(1):59-69.

19. Jiapeng Tang, Zhenqing Qian, Hui Wu*. Enhancing cordycepin production in liquid static cultivation of Cordyceps militaris by adding vegetable oils as the secondary carbon source. Bioresource Technology. 2018. 268:60-67. (JCR Q1)

20. Bing Huang, Hao Yang, Guochen Fang, Xing Zhang, Hui Wu*, Zhimin Li*, Qin Ye. Central pathway engineering for enhanced succinate biosynthesis from acetate in Escherichia coli. Biotechnology and Bioengineering. 2018. 115:943-954. (*Co-corresponding) (JCR Q2)

21. Wei Li^#, Hui Wu^#, Mai Li, Ka-Yiu San*. Effect of NADPH availability on free fatty acid production in E. coli. Biotechnology and Bioengineering. 2018. 115:444-452. (JCR Q2)

22. Han Liu, Guochen Fang, Hui Wu*, Zhimin Li*, Qin Ye. L-cysteine production in Escherichia coli based on rational metabolic engineering and modular strategy. Biotechnology Journal. 2018. 13:e1700695. (*Co-corresponding) (JCR Q1)

23. Qing Li#, Bing Huang#, Qiaofei He, Jingxian Lu, Xun Li, Zhimin Li, Hui Wu*, Qin Ye. Production of succinate from simply purified crude glycerol by engineered Escherichia coli using two-stage fermentation. Bioresources and Bioprocessing. 2018. 5:41. (Feature Article) (JCR Q2)

24. Jiaqi Jiang, Bing Huang, Hui Wu*, Zhimin Li*, Qin Ye. Efficient 3-hydroxypropionic acid production from glycerol by metabolically engineered Klebsiella pneumoniae. Bioresources and Bioprocessing. 2018. 5:34. (*Co-corresponding) (JCR Q2)

25. Qing Li, Bing Huang, Hui Wu*, Zhimin Li, Qin Ye. Efficient anaerobic production of succinate from glycerol in engineered Escherichia coli by using dual carbon sources and limiting oxygen supply in preceding aerobic culture. Bioresource Technology. 2017. 231:75-84. (JCR Q1)

26. Xing Zhang^#, Hui Wu^#, Bing Huang, Zhimin Li*, Qin Ye. One-pot synthesis of glutathione by a two-enzyme cascade using a thermophilic ATP regeneration system. Journal of Biotechnology. 2017. 241:163-169. (^# First two authors contributed to this paper equally) (JCR Q2)

27. Qing Li^#, Hui Wu^#, Zhimin Li, Qin Ye*. Enhanced succinate production from glycerol by engineered Escherichia coli strains. Bioresource Technology. 2016. 218:217-223. (^# First two authors contributed to this paper equally) (JCR Q1)

28. Yunjie Li, Bing Huang, Hui Wu*, Zhimin Li*, Qin Ye, Yi-Heng Percival Zhang. Production of succinate from acetate by metabolically engineered Escherichia coli. ACS Synthetic Biology. 2016. 5:1299-1307. (*Co-corresponding) (JCR Q1)

29. Jianhua Yang, Wei Li, Dezheng Wang, Hui Wu*, Zhimin Li*, Qin Ye. Characterization of bifunctional L-glutathione synthetases from Actinobacillus pleuropneumoniae and Actinobacillus succinogenes for efficient glutathione biosynthesis. Applied Microbiology and Biotechnology. 2016. 100(14):6279-6289. (*Co-corresponding) (JCR Q1)

30. Jing Zhang, Cong Quan, Cheng Wang, Hui Wu*, Zhimin Li*, Qin Ye. Systematic manipulation of glutathione metabolism in Escherichia coli for improved glutathione production. Microbial Cell Factories. 2016. 15:38. (*Co-corresponding) (JCR Q1)

31. Dezheng Wang, Cheng Wang, Hui Wu*, Zhimin Li*, Qin Ye. Glutathione production by recombinant Escherichia coli expressing bifunctional glutathione synthetase. Journal of Industrial Microbiology and Biotechnology. 2016. 43:45-53. (*Co-corresponding) (JCR Q2)

32. Cheng Wang, Jing Zhang, Hui Wu*, Zhimin Li*, Qin Ye. Heterologous gshF gene expression in various vector systems in Escherichia coli for enhanced glutathione production. Journal of Biotechnology. 2015. 214:63-68. (*Co-corresponding). (JCR Q2)

33. Hui Wu, Leepika Tuli, George N. Bennett, Ka-Yiu San*. Metabolic transistor strategy for controlling electron transfer chain in Escherichia coli. Metabolic Engineering. 2015. 28:159-168. (JCR Q1)

34. Hui Wu, George N. Bennett, Ka-Yiu San*. Metabolic control of respiratory levels in coenzyme Q biosynthesis-deficient Escherichia coli strains leading to fine-tune aerobic lactate fermentation. Biotechnology and Bioengineering. 2015. 112(8):1720-1726. (JCR Q2)

35. Hui Wu, Jane Lee, Mukund Karanjikar, Ka-Yiu San*.  Simultaneous utilization of glucose and mannose from woody hydrolysate  for free fatty acid production by metabolically engineered Escherichia coli. Bioresource Technology. 2015. 185:431-435. (JCR Q1)

36. Hui Wu, Mukund Karanjikar, Ka-Yiu San*. Metabolic engineering of Escherichia coli for efficient free fatty acid production from glycerol. Metabolic Engineering. 2014. 25:82-91. (JCR Q1)

37. Hui Wu, Ka-Yiu San*. Efficient odd numbered straight-chain free fatty acid production by metabolically engineered Escherichia coli. Biotechnology and Bioengineering. 2014. 111(11):2209-2219. (JCR Q2)

38. Yane Luo*, Tao Zhang, Hui Wu*. The transport and mediation mechanisms of the common sugars in Escherichia coli. Biotechnology Advances. 2014. 32:905-919. (JCR Q1)

39. Hui Wu, Jane Lee, Mukund Karanjikar, Ka-Yiu San*. Efficient free fatty acid production from woody biomass hydrolysate in metabolically engineered Escherichia coli. Bioresource Technology. 2014. 169:119-125. (JCR Q1)

40. Hui Wu, Ka-Yiu San*. Engineering Escherichia coli for odd straight medium chain free fatty acid production. Applied Microbiology and Biotechnology. 2014. 98(19):8145-8154. (JCR Q1)

41. Hui Wu, Qing Li, Zhi-min Li*, Qin Ye*. Succinic acid production and CO₂ fixation using a metabolically engineered Escherichia coli in a bioreactor equipped with a self-inducing agitator. Bioresource Technology. 2012. 107:376-384. (JCR Q1)

42. Yuan Liu^♯, Hui Wu^♯, Qin Li, Xuwei Tang, Zhi-min Li*, Qin Ye. Process development of succinic acid production by Escherichia coli NZN111 using acetate as an aerobic carbon source. Enzyme and Microbial Technology. 2011. 49(5):459-64. (^♯ First two authors contributed to this paper equally) (JCR Q2)

43. Hui Wu, Zhi-min Li, Li Zhou, Jingli Xie, Qin Ye*. Enhanced anaerobic succinic acid production by Escherichia coli NZN111 aerobically grown on gluconeogenetic carbon sources. Enzyme and Microbial Technology. 2009. 44:165-169. (JCR Q2)

44. Hui Wu, Zhi-min Li, Li Zhou, Qin Ye*. Improved succinic acid production in the anaerobic culture of an Escherichia coli pflB ldhA double mutant as a result of enhanced anaplerotic activities in the preceding aerobic culture. Applied and Environmental Microbiology. 2007. 73:7837-7843. (JCR Q1)

1. 副主编.《生物基可降解材料的研究与应用》. 上海科学出版社. 2022.

2. Qiang Fei, Haritha Meruvu, Hui Wu, Rongzhan Fu，Chapter 12: Biological Conversion of Natural Gas in Direct Natural Gas Conversion to Value-Added Chemicals, 512 Pages, ISBN 9780367077938, 2020, CRC Press.

     3. Qin Ye*, Zhimin Li, Hui Wu. Principle and performance of gas self-inducing reactors and applications to biotechnology. Bioreactor Engineering Research and Industrial Applications II, Advances in Biochemical Engineering/Biotechnology, Volume 152, 2016. ISBN: 978-3-662-48346-6 (Print), 978-3-662-48347-3 (eBook). Springer, Berlin, Heidelberg, Germany.